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OVERVIEWCo-immunoprecipitation requires intact interaction among proteins in order to co-immunoprecipitate protein complexes with the target protein. To keep the interactions…

Note: Sodium hydroxide (NaOH) Pellets can be purchased from any of the following suppliers. You must visit the supplier’s product…

The molarity and normality of the Sodium hydroxide (NaOH) solution are the same which means 1M NaOH solution is also…

Here we have described a procedure to prepare 10 % sodium hydroxide (NaOH) solution. A 10% NaOH solution can be prepared by dissolving 10 g of NaOH in water to a final volume of 100 ml. Since dissolution of NaOH in water is a highly exothermic reaction, extreme care must be taken while preparing the solution.

OVERVIEW A 1N solution of Sodium hydroxide (NaOH) solution can easily be prepared by diluting if you have already concentrated…

The normality and molarity of the Sodium hydroxide (NaOH) solution are the same. This means the 10N solution of sodium…

Note: The 10N (or 10M) Sodium hydroxide (NaOH) solution can be purchased from the following suppliers. You must visit the…

NOTES Sodium Hydroxide (NaOH)  Molarity vs Normality of Sodium Hydroxide (NaOH) Solution SOLUTION PREPARATION Preparation of 10 M Sodium Hydroxide…

Glycerol (C3H8O3) is an organic compound of three elements: Carbon, Hydrogen, and Oxygen. The molecular weight of Glycerol is 92.0943 which can be calculated by adding up the total weight (atomic weight multiplied by their number) of all its elements.

As the name suggests, co-immunoprecipitation employs specific antibodies to sequester a protein of interest together with its interacting partners from…

OVERVIEW The 10x Tris-Glycine Transfer Buffer can be used to transfer proteins from polyacrylamide gel to nitrocellulose or PVDF membranes…

Note: The 10x SDS-PAGE running buffer can be purchased from the following suppliers. You must visit the supplier’s product page…

OVERVIEW Tris-Glycine-SDS running buffer is commonly used to resolve proteins in SDS-polyacrylamide gel. A concentrated buffer is usually prepared that…

Protein blotting refers to transferring and immobilizing the proteins on a solid membrane support. The immobilized proteins, which are accessible…

Tris (C4H11NO3) is an organic compound of four elements: Carbon, Hydrogen, Nitrogen, and Oxygen. The Tris hydrochloride (C4H11NO3.HCl) also contains one molecule of Hydrogen chloride. The molecular weight of Tris hydrochloride is 157.59644 which can be calculated by adding up the total weight (atomic weight multiplied by their number) of all its elements.